By S. P. Borriello (auth.), S. Peter Borriello PhD. (eds.)
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Extra info for Antibiotic Associated Diarrhoea and Colitis: The role of Clostridium difficile in gastrointestinal disorders
5 grams by mouth every 6 hours for 7 to 14 days. There are therapy such Lactobacillus agents. , 1980 ). Management of relapse of C. difficile-induced diarrhea. C. , 1980 b ) symptoms of C. associated diarrhea during treatment with vancomycin (or, difficileoccasion- ally, bacitracin or metronidazole) may develop relapse of disease from several days to 5 or 6 weeks after discontinuation of vancomycin. It is important to realize that a significant proportion of patients with C. difficile-induced diarrhea will have resolution of symptoms as a consequence of discontinuation of the inciting agent.
1982). le cytotox in detec1978, George, 1979). The procedu re used for C. diffici . follows as is ory laborat our in tion in stool specime ns d Salt solutio n An approxi mate 1:10 suspens ion of stool in Hanks Balance ged at 12,000 rpm (Hanks BSS) is prepare d, thoroug hly mixed and centrifu ly removed using a for 30 minutes at 4°C. 45 ~ filter. 1 ml aliquot of the superna tant is then added to ion on a roller incubat ht overnig after d recorde is xicity monola yer. Cytoto the presenc e and tive distinc is drum at 37°C.
Assay for of diarrhea to specific therapy directed An excellent description of the tissue culture cytotoxin has been publ ished by one of the groups that discovered the role of C. , 1979a); this assay is recommended to clinical laboratories. , 1980 ). , 1980 and Moskovitz and Bart lett, 1981 (or exacerbation of inflammatory bowel disease) due to C. difficile. In contrast are to adults, asymptomatic young children or infants frequently intestinal carriers of C. , 1981 ). a and Because of this, positive cytotoxin assay is of limited diagnostic value in an infant young child who has diarrhea; a negative assay, of course, indicates that C.
Antibiotic Associated Diarrhoea and Colitis: The role of Clostridium difficile in gastrointestinal disorders by S. P. Borriello (auth.), S. Peter Borriello PhD. (eds.)